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KMID : 0391319920020020143
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Korean Journal of Biological Response Modifiers
1992 Volume.2 No. 2 p.143 ~ p.150
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Identification of Specific Interferon- Response Region (GRR) Binding Nuclear Protein in CML Cells Stimulated by Recombinant Human IFN-
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양일호
김동욱/이종욱/진종률/한치화/민우성/박종원/김춘추/김원일
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Abstract
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interferon-gamma (IFN-γ) induces expression of the high affinity receptor (FcγRI) for IgG in myeloid series and alters binding of nuclear proteins to transcriptional enhancers of FcγRI gene. Bandshift assay was done with IFN-γresponse region
(GRR)
of FcγRI gene and DNA-binding proteins of U937 cells which pretreated with or without IFN-γ As a result, a specific band located at 200 kd could be induced in U937 cells preincubated with IFN-γ-untreated U937 cells. One ng of of GRR
oligonucleotide
and 10 ㎍ of U397 DNA-binding protein which was incubated by 1,000 units IFN-γ/ml for 1 hour were necessary for the induction of the specific band in a 20 μl of bandshift assay. Peripheral mononuclear cells (PMNC) were incubated with IFN-γin
vitro
and bandshift assay was done, and those two samples also showed the same band as those which were found in IFN-γtreated U937 cells Among three patients with chronic myelogenous leukemia who had been administered with 3×10E6 unit of IFN-γdaily
(one in
hematological remission, the others without clinical responses). the same sized specific band (200 kd) was detected in responder, but smaller ones (180 kd) were found in monresponders. Our preliminary data suggest that bandshift assay using GRR
oligonucleotide could be a useful indicator to predict whether IFN-γwould be effective for the patient with CML or not.
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